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Lecturer(s)
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Koldušková Klára, Ing.
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Lysák Daniel, Doc. MUDr. Ph.D.
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Hajšmanová Zdeňka, MUDr.
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Lavičková Alena, MUDr.
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Šlechtová Jitka, MUDr.
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Dokoupilová Eliška, Mgr.
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Krupičková Jánská Eva, MUDr.
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Course content
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Lectures 1. Blood and bone marrow, function of cellular blood elements in peripheral blood, nomenclature. Physiological values of blood elements. Physiology of hematopoiesis, proliferation, differentiation and maturation of individual hematopoietic lineages. 2. Development, function and destruction of erythrocytes (hemoglobin synthesis and function, iron metabolism, breakdown products of Hb during intravascular and extravascular hemolysis). 3. Development and function of leukocytes, morphological differentiation of leukocytes. 4. Platelet function, primary hemostasis. 5. Blood clotting and its stages. 6. Mechanisms ensuring hemostasis, coagulation and fibrinolytic system, system of natural inhibitors. 7. Blood count and hemocoagulation screening tests. 8. Blood particle analyzers and coagulometers, screening and special coagulation tests, calibration and validation of hemocoagulation methods. 9. Laboratory diagnosis of blood diseases and disorders of hemostasis, differential diagnosis. 10. Pathophysiology of blood diseases and disorders of hemostasis. 11. Tumor disorders of hematopoietic cells - leukemia, myelodysplastic syndrome, myeloproliferative disease, lymphoproliferative disease. A Lab exercise 1. Principles of proper laboratory work. 2. Examination of the blood picture on an automatic hematological analyzer. 3. Examination of peripheral blood smears microscopically, determination of the number of blood elements in the counting chamber. 4. Microscopic examination of bone marrow smears. 5. Investigation of hemolysis including osmotic resistance of erythrocytes. 6. Examination of platelet aggregation on an aggregometer. 7. Examination of basic coagulation tests - prothrombin time and activated partial thromboplastin time on a coagulometer. 8. Determination of factors of the plasma system of blood coagulation. 9. Determination of inhibitors of the plasma system of blood coagulation. 10. Hemoglobin electrophoresis, determination of the von Willebrand factor by the LIA method, evaluation of the outputs of hematology analyzers, including interferences. 11. Cytochemical staining methods.
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Learning activities and teaching methods
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Laboratory work, Lecture with visual aids, Practicum
- Contact hours
- 77 hours per semester
- Preparation for comprehensive test (10-40)
- 20 hours per semester
- Preparation for an examination (30-60)
- 35 hours per semester
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| learning outcomes |
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| Knowledge |
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| explains the physiology of hematopoiesis and blood coagulation and, on this basis, clarify the essence of malignant and non-malignant blood diseases and blood coagulation disorders |
| explains the principles of laboratory diagnosis of blood diseases and coagulation disorders and interprets the possibilities and limits of instrumental blood picture analysis and coagulometers |
| Skills |
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| performs an instrumental analysis of the blood picture |
| makes a smear of peripheral blood and bone marrow and evaluates it microscopically |
| performs instrumental screening coagulation tests and evaluates them |
| controls basic operation and daily operation of analyzers and coagulometers |
| calibrates the instruments and sets the daily norm |
| differentiates physiological from pathological results and performs basic differential diagnosis of blood diseases |
| Competences |
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| N/A |
| N/A |
| teaching methods |
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| Knowledge |
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| Lecture |
| Interactive lecture |
| Skills |
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| Laboratory work |
| Competences |
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| Lecture with visual aids |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Oral exam |
| Test |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Oral exam |
| Test |
| Skills demonstration during practicum |
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Recommended literature
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Web Laboratorní sekce České hematologické společnosti ČLS JEP - obecná doporučení. .
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BAIN, Barbara J. Blood Cells: A Practical Guide. Sixth Edition.. John Wiley & Sons Ltd., 2022. ISBN 9781119820277.
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PENKA, Miroslav, TESAŘOVÁ, Eva a kolektiv. Hematologie a transfuzní lékařství I - Hematologie.. Praha : Grada, 2011. ISBN 978-80-247-3459-0.
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ŠEBEK, Jaroslav. Hematologie a laboratorní technika.. Praha : Galén, 2022. ISBN 978-80-7492-631-0.
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ŠKORŇOVÁ, Ingrid, SLAVÍK, Luděk a kol. Hemostáza, laboratorní metody, jejich využití a interpretace ve vybraných klinických situacích. 2. vydání.. Olomouc: Univerzita Palackého v Olomouci, 2021. ISBN 978-80-244-6014-7.
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VYDRA, Jan, NOVÁK, Jan, LAUERMANNOVÁ, Marie a kol. Hematologie v kostce. Druhé, aktualizované vydání.. Praha: Mladá fronta, 2019. ISBN 978-80-2045-140-8.
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ZIMA, Tomáš. Laboratorní diagnostika. Třetí, doplněné a přepracované vydání. Praha : Galén, 2013. ISBN 978-80-7492-062-2.
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