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Lecturer(s)
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Course content
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Lectures 1. The immune system. 2. Non-specific and specific immunity. 3. Cells of the immune system. 4. Humoral immunity. 5. Barrier functions of the body and mucosal immunity. 6. Cytokines and inflammation. 7. Complement, antigen presentation, HLA. 8. Flow cytometry. 9. T-lymphocytes, Th1/Th2/Tc reaction. 10. B-lymphocytes. 11. Immunoglobulins. An antibody-based immune response. 12. Regulation of the immune system. 13. Repetition. A Lab exercise 1. Occupational safety and fire protection, legal regulations. 2. Accuracy and correctness of laboratory methods. 3. Cells of the immune system - differential budget of leukocytes, microscopy. 4. Agglutination and precipitation methods. 5. Nephelometric determination of serum immunoglobulins. 6. ELISA 7. CIK - proof of circulating immunocomplexes. 8. Flow cytometry - determination of phenotyping of T and B lymphocytes. 9. Flow cytometry - determination of oxidative burst. 10. Flow cytometry - determination of basophil activation test. 11. Flow cytometry - blastic transformation of lymphocytes. 12. Repetition 13. Credit test
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Learning activities and teaching methods
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- Preparation for comprehensive test (10-40)
- 35 hours per semester
- Contact hours
- 65 hours per semester
- Preparation for laboratory testing; outcome analysis (1-8)
- 5 hours per semester
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| prerequisite |
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| Knowledge |
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| specific knowledge prerequisites are not required |
| Skills |
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| specific skill prerequisites are not required |
| Competences |
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| N/A |
| N/A |
| learning outcomes |
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| Knowledge |
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| defines basic immunological terms and characterizes individual components of non-specific and specific immunity |
| will explain the principles of laboratory methods for monitoring humoral and cellular immunity |
| Skills |
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| observes the principles of work safety and fire protection in the immunology laboratory |
| under supervision, performs protein identification including Ig nephelometry and turbidimetry and detection of circulating immunocomplexes |
| using the flow cytometry method, determines the phenotyping of T and B lymphocytes, basophil activation test, lymphocyte blast transformation and oxidative burst under supervision |
| controls the principle of ELISA immunochemical methodology using an enzyme to mark an antigen or an antibody |
| Competences |
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| N/A |
| N/A |
| teaching methods |
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| Knowledge |
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| Lecture |
| Skills |
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| Laboratory work |
| Competences |
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| Lecture |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Test |
| Continuous assessment |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Continuous assessment |
| Test |
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Recommended literature
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Česká společnost alergologie a klinické imunologie.
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International Consensus on ANA Patterns.
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The Journal of Applied Laboratory Medicine: Oxford: Oxford Academic, 2016-. ISSN 2576-9456.
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BARTŮŇKOVÁ, Jiřina, PAULÍK, Milan a kol. Vyšetřovací metody v imunologii. 2., přepracované a doplněné vydání.. Praha : Grada, 2011. ISBN 978-80-247-3533-7.
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BARTŮŇKOVÁ, Jiřina, ŠEDIVÁ, Anna a kol. Imunodeficience. 3., přepracované a doplněné vydání.. Praha : Grada, 2021. ISBN 978-80-271-1273-9.
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HOŘEJŠÍ, Václav, Jiřina BARTŮŇKOVÁ, Tomáš BRDIČKA a Radek ŠPÍŠEK. Základy imunologie. 6., aktualizované vydání.. Praha: Stanislav Juhaňák - Triton, 2017. ISBN 978-80-7553-250-3.
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KREJSEK, Jan, ANDRÝS, Ctirad a Irena KRČMOVÁ. Imunologie člověka.. Hradec Králové: Garamon s.r.o., 2016. ISBN 978-80-86472-74-4.
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VLAS, Tomáš. ELISA, LEIA, FEIA, Biočipy..
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VLAS, Tomáš. Nepřímá imunofluorescence..
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VLAS, Tomáš. Průtoková cytometrie..
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VLAS, Tomáš. Vyšetření komplementu, nefelometrie + turbidimetrie..
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