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Lecturer(s)
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Course content
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Lab excercises 1. Optics 1 - absorption spectral methods 2. Optics 2 - emission spectral methods 3. Optics 3 - non-spectral methods 4. Electrochemistry 1 - division and types of electrodes 5. Electrochemistry 2 - methods based on electrode action and measurement of electrical properties 6. Osmometry 7. Immunochemistry 1 - immunoprecipitation methods 8. Immunochemistry 2 - immunoanalytical methods 9. Chromatography 1 - sample preparation methods, extraction methods 10. Chromatography 2 - liquid chromatography 11. Chromatography 3 - gas chromatography 12. Electrophoresis 1 - planar and vertical methods 13. Electrophoresis 2 - capillary methods 14. Overview of automatic analyzers 15. Development and characteristics of modern laboratory equipment Lab exercises 1. Safety of work in the laboratory 2. Practical laboratory calculations 3. Preparation of solutions and reagents 4. Accuracy and correctness of automatic pipettes 5. Photometric determination of the concentration of copper salts 6. Spectrophotometric determination of protein concentration by biuret reaction 7. pH-metry of acid and base solutions 8. Neutralization potentiometric titrations 9. Conductometric determination of cell suspension conductivity 10. Qualitative determination of amino acids by thin-layer chromatography 11. RNA extraction on solid phase from buccal mucosa cells 12. Agarose electrophoresis of nucleic acids 13. Vertical electrophoresis of proteins on a polyacrylamide gel 14. Maintenance and quality control of laboratory equipment 15. Internal and external quality control of laboratories
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Learning activities and teaching methods
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- Contact hours
- 75 hours per semester
- Preparation for comprehensive test (10-40)
- 20 hours per semester
- Preparation for an examination (30-60)
- 30 hours per semester
- Preparation for laboratory testing; outcome analysis (1-8)
- 5 hours per semester
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| prerequisite |
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| Knowledge |
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| no specific knowledge prerequisites are required |
| Skills |
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| no specific skill prerequisites are required |
| Competences |
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| N/A |
| learning outcomes |
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| Knowledge |
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| describes the principle of the function of the devices in the clinical laboratory |
| describes the physical and physico-chemical principles of analytical methods |
| lists the design elements of instrumentation and the elements of daily, weekly and monthly maintenance of the analyzer |
| Skills |
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| observes the principles of safety and health protection when working in the clinical laboratory |
| keeps documentation on measurement results |
| ndependently uses instrumentation |
| controls standard technical operating procedures (SOPT), work instructions for the operation and maintenance of devices |
| keeps operational logs |
| checks the activities and settings of individual analyzer components (photometer check, ISE check, cell blank, pipettor settings) |
| channels error messages and status messages of the automatic analysis system. |
| Competences |
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| N/A |
| N/A |
| teaching methods |
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| Knowledge |
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| Lecture with visual aids |
| Skills |
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| Laboratory work |
| Competences |
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| Lecture with visual aids |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Oral exam |
| Test |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Oral exam |
| Skills demonstration during practicum |
| Test |
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Recommended literature
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DASTYCH, Milan, ČERVENÝ, Ladislav a Ivo NAJMAN. English for Laboratory Technicians - Skripta LFMU.. Brno: Lékařská fakulta Masarykovy univerzity v Brně. ISBN 978-80-210-4241-4.
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DASTYCH, Milan, Ludmila BOURKOVÁ, Marta HANÁKOVÁ, Jana JURÁNKOVÁ, Eva TESAŘOVÁ, Iveta VALÁŠKOVÁ a Jiřina ZAVŘELOVÁ. Instrumentální technika. Obor zdravotní laborant. 2. vyd.. Brno: Masarykova univerzita, 2014. ISBN 978-80-210-7103-2.
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