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Lecturer(s)
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Karlíková Marie, RNDr. Ph.D.
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Course content
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Lectures 1. General principles of immunoassay. 2. Basic substances, separation techniques. 3. Types of immunoanalytical methods, advantages/disadvantages of individual types of analysis. 4. Specific preanalytical problems in immunoanalysis. 5. Basic types of instruments for immunoanalysis. Manual pipetting methods, semi-automatic devices, automatic devices. 6. Multiplex analysis. 7. Quality parameters of immunoassay. Internal and external quality control. Evidence of selected ions. 8. Main principles of interpretation of results. 9. Overview of the clinical application of immunoanalytical methods. 10. - 12. Use and specifics of application in endocrinology. A Lab exercise 1. - 5. Radioimmunoanalysis. 6. - 8. Chemiluminescence. 9. - 11. Evaluation of internal and external quality control. 12. Credit test.
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Learning activities and teaching methods
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Lecture, Practicum
- Preparation for comprehensive test (10-40)
- 20 hours per semester
- Contact hours
- 24 hours per semester
- Preparation for laboratory testing; outcome analysis (1-8)
- 8 hours per semester
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| prerequisite |
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| Knowledge |
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| explain the term immunocomplex |
| to describe the body's immune response based on the production of antibodies |
| Skills |
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| to distinguish polyclonal from monoclonal antibodies |
| Competences |
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| N/A |
| N/A |
| N/A |
| learning outcomes |
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| Knowledge |
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| will describe highly specific and highly sensitive (nmol - pmol) procedures in the analysis of biological material |
| Skills |
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| has basic laboratory habits in the field of immunoanalysis |
| works with selected laboratory instruments |
| evaluates examination quality parameters |
| Competences |
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| N/A |
| N/A |
| teaching methods |
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| Knowledge |
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| Lecture with visual aids |
| Skills |
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| Laboratory work |
| Skills demonstration |
| Competences |
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| Lecture with visual aids |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Test |
| Skills |
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| Skills demonstration during practicum |
| Continuous assessment |
| Competences |
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| Skills demonstration during practicum |
| Continuous assessment |
| Test |
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Recommended literature
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BARTOŠ, Vladimír, ŠAFARČÍK, Kristián, KARLÍKOVÁ, Marie, TOPOLČAN, Ondřej, KUČERA, Radek a Jindra WINDRICHOVÁ. Principy imunoanalytických metod pro mediky.. 2013.
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Wild D. (Ed.). The Immunoassay Handbook Theory and Applications of Ligand Binding, ELISA and Related Techniques. Fourth Edition. Elsevier, 2013. ISBN 978-0-08-097037-0.
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ZIMA, Tomáš. Laboratorní diagnostika. Třetí, doplněné a přepracované vydání.. Praha : Galén, 2013. ISBN 978-80-7492-062-2.
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