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Lecturer(s)
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Šebek Jaroslav, Mgr. DiS.
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Course content
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HEMATOLOGY - 3 weeks (120 hours) Content content of practice - internal and external quality control in the hematology laboratory - receipt of material, organization of operation, SOP, registration and dispatch of results - taking capillary blood, making a peripheral blood smear - determination of blood count values - differential budget, morphology of stained blood cells in normal and pathological conditions - basic cytochemical examinations - prothrombin test - APTT - clotting - bleeding - examination of fibrinogen and fibrin products - budget of stained smears from sternal puncture
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Learning activities and teaching methods
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- Practical training (number of hours)
- 120 hours per semester
- Presentation preparation (report) (1-10)
- 10 hours per semester
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| prerequisite |
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| Knowledge |
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| to explain the physiology of hematopoiesis and blood clotting |
| to explain the principles of laboratory diagnosis of blood diseases and coagulation disorders and interpret the possibilities and limits of the instrumental analysis of the blood picture and coagulometers |
| Skills |
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| to perform instrumental analysis of the blood picture, make a smear of peripheral blood and bone marrow and evaluate it microscopically |
| to perform instrumental screening coagulation tests and evaluate them |
| to control the basic operation and daily operation of analyzers and coagulometers, calibrate the devices and establish the daily norm |
| to distinguish physiological from pathological results and perform basic differential diagnosis of blood diseases |
| Competences |
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| N/A |
| N/A |
| N/A |
| learning outcomes |
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| Knowledge |
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| defines the content of the laboratory manual, operating and hygiene regulations of the hematology laboratory |
| Skills |
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| independently performs basic hematological examinations |
| determines blood count values |
| makes a peripheral blood smear |
| examines fibrinogen |
| carries out the budget of colored smears |
| determines leukocyte morphology |
| stains and counts reticulocytes |
| performs basic cytochemical examinations and basic and special coagulation examinations |
| Competences |
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| N/A |
| N/A |
| N/A |
| teaching methods |
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| Knowledge |
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| Clinical practice under supervision |
| Skills |
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| Clinical practice under supervision |
| Competences |
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| Clinical practice under supervision |
| assessment methods |
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| Knowledge |
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| Continuous assessment |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Skills demonstration during practicum |
| Continuous assessment |
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Recommended literature
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Věstník Ministerstva zdravotnictví 8/2003 Metodické opatření 5. Řešení problematiky infekce HIV/AIDS.
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Vyhláška č. 143/2008 Sb., o stanovení bližších požadavků pro zajištění jakosti a bezpečnosti lidské krve a jejích složek (vyhláška o lidské krvi), ve znění pozdějších předpisů.
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Zákon č. 378/2007 Sb., zákon o léčivech.
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FABER, Edgar. Základy hematologické diagnostiky. Druhé přepracované vydání.. Praha: Mladá fronta, 2015. ISBN 978-80-204-3742-6.
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PENKA, Miroslav a Eva SLAVÍČKOVÁ. Hematologie a transfuzní lékařství I. Hematologie.. Praha : Grada, 2011. ISBN 978-80-247-3459-0.
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ŠEBEK, Jaroslav. Hematologie a laboratorní technika.. Praha : Galén, 2022. ISBN 978-80-7492-631-0.
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