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Lecturer(s)
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Course content
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CLINICAL BIOCHEMISTRY - 3 weeks (120 hours) Content content of practice - internal and external quality control of examinations, registration and dispatch of results, organization of operations - preparatory and auxiliary work - disinfection, sterilization, cleaning of laboratory equipment - urine examination - sampling systems, amount, color, odor, turbidity, foam, biochemical composition (proteins, carbohydrates, ketone bodies, bile pigments, bilirubin, urobilinogen, urobilin, hemoglobin, phenylpyruvic acid, urinary sediment - blood cells, epithelia, cylinders, crystals) - control of the pre-analytical phase - stool examination - determination of serum proteins - urea, creatinine, creatine, uric acid in serum - serum amino acids - selected immunochemical examinations - ABR examination - toxicological examinations TRANSFUSION SERVICE - 1 week (40 hours) Content content of practice - registration and dispatch of results, organization of operation, SMJ, SVP and work with LIS - laboratory examination of blood donors - laboratory evidence of blood groups and blood compatibility - examination of antibodies - examination of leukocyte and platelet antigens - immunohematological examination in hemolytic disease of the newborn - examination to reduce the risk of transmission of infectious diseases through transfusion preparations CLINICAL GENETICS - 1 week (40 hours) Content content of practice - internal and external quality control in the genetic laboratory - organization of operation, registration and dispatch of results - genetic analysis methods, inheritance type estimates - tissue cultures in vitro and in situ, construction and structure of chromosomes - striping methods, fluorescence in situ hybridization modern methods of chromosomal analyzes (FISH, CGH) - cytogenetic methods - PRINS, mFISH, mBAND FISH, SKY, CGH, array CGH - analysis of chromosomal aberrations - collection of biological material - DNA isolation - salting out method - PCR, electrophoresis, restriction digestion, real time PCR - genotoxic tests
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Learning activities and teaching methods
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- Practical training (number of hours)
- 200 hours per semester
- Presentation preparation (report) (1-10)
- 10 hours per semester
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| prerequisite |
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| Knowledge |
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| to characterize the changes in the chemical composition of the organism that can be used to determine the diagnosis, prognosis and follow the course of the disease |
| to describe the methodology of taking biological material for genetic examination |
| to characterize individual laboratory investigation methods used in cytogenetics and molecular genetics |
| to describe the applicable legislation and the basics of the quality management system in transfusion service facilities |
| to enumerate the principles of good manufacturing practice, specify transfusion preparations and their indications and characterize procedures ensuring the safety of hemotherapy |
| Skills |
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| compare measured values with reference values |
| to perform qualitative and quantitative urine tests and serological tests |
| to keep protocols and control instrumentation in the clinical biochemistry laboratory |
| to process, culture and stain material for cytogenetic examination |
| to distinguish normal and pathological karyotype in prenatal and postnatal diagnosis and oncocytogenetics |
| to perform blood group tests in the ABO system and Rh D antigens |
| Competences |
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| learning outcomes |
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| Knowledge |
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| defines the content of the laboratory manual, operating and hygiene regulations of the clinical-biochemical laboratory, the workplace of the transfusion service and the laboratory of clinical genetics |
| Skills |
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| independently performs routine clinical and biochemical examinations, examination of blood groups and blood donors, examination of antibodies and antigens, examination of hemolytic disease of the newborn |
| describes the structure and structure of chromosomes |
| determines the types of inheritance |
| isolates DNA |
| controls the procedure of genotoxic tests |
| Competences |
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| teaching methods |
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| Knowledge |
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| Clinical practice under supervision |
| Skills |
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| Clinical practice under supervision |
| Competences |
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| Clinical practice under supervision |
| assessment methods |
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| Knowledge |
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| Individual presentation at a seminar |
| Continuous assessment |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Continuous assessment |
| Skills demonstration during practicum |
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Recommended literature
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DASTYCH, Milan, BREINEK, Petr a kol. Klinická biochemie: bakalářský obor Zdravotní laborant. 3. vydání, 1. dotisk. Brno: Masarykova univerzita, 2021. ISBN 978-80-210-7788-1.
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DASTYCH, Milan, Ludmila BOURKOVÁ, Marta HANÁKOVÁ, Jana JURÁNKOVÁ, Eva TESAŘOVÁ, Iveta VALÁŠKOVÁ a Jiřina ZAVŘELOVÁ. Instrumentální technika. Obor zdravotní laborant. 1. vyd.. Brno: Masarykova univerzita, 2014. ISBN 978-80-210-7103-2.
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KOČÁREK, Eduard, Martin PÁNEK a Drahuše NOVOTNÁ. Klinická cytogenetika I: vyšetřovací metody v klinické cytogenetice.. Praha: Karolinum, 2006. ISBN 80-246-1069-8.
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PENKA, Miroslav, TESAŘOVÁ, Eva a kol. Hematologie a transfuzní lékařství II. Transfuzní lékařství.. Praha : Grada, 2012. ISBN 978-80-247-3460-6.
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