Lectures: 1. Toxicology. Introduction, history, sub-disciplines (clinical, industrial, experimental, etc..). Resources and applications of toxicology. 2. Poisons. Definition, classification, labeling, dose-effect, toxicokinetics (entry, metabolism, excretion). 3. Types of materials. Sampling dates, treatment before analysis (isolation, extraction, separation, mineralization etc.). 4. Toxicological examination. The reasons, known - unknown exposure, screening, biological exposure tests - BET, the choice of the investigated substance according to the time of exposure, interpretation (maximum permissible limit, NPK). 5. Analytical techniques. Principles of quantitative methods (spectrophotometry, chromatography, immunochemical methods etc.). 6. Poisoning by agents blocking the oxygen transfer. 7. Poisoning by alcohols (ethanol, methanol, ethylene glycol). 8. Poisoning by heavy metals, solvents, "-icides" (herbicides, pesticides, fungicides), rarer types of poisoning. 9. Poisoning by substances from the life and domestic environment (plants, animals, chemist goods, medicinal products). 10. Drug levels monitoring (TDM), the pharmacokinetic evaluation. 11. Drugs and abused substances. Classification of groups and effects, screening, conventional drugs - new drugs. Laboratory practicals: 1. Principles on the safety in the toxicological laboratory, instruments and equipment. 2. - 3. The biological material - types, quantity, method of preservation, processing time, documentation. 4. - 5. Adjustments of samples, deproteinization, extraction, hydrolysis, isolation mineralization. 6. Analytical methods in toxicology - immunochemical. 7. Analytical Methods in toxicology - chromatographic (TLC, GC, HPLC). 8. Analytical methods in toxicology - spectrophotometric (AAS, UV-VIS, IR). 9. Organization of work in a toxicological laboratory, toxicological analysis in the mode POCT, excursion to the lab. 10. Issues of the forensic toxicology (collection and preservation of material, accredited methods, apparatus equipment of the laboratory). 11. Written credit exam.
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Balíková, M. Forenzní a klinická toxikologie. Galén, Praha 2004.
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Katzung, B., G. Basic and Clinical Pharmacology. Lange medical books. McGrawn-Hill, 2007.
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Lincová, D., Farghali, H. Základní a aplikovaná farmakologie. Galén, Praha 2006.
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Racek, J. a kol. Klinická biochemie. Galén, Praha, Karolinum, 2006.
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