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Lecturer(s)
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Pavelka Jaroslav, Mgr. Ph.D.
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Course content
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1. Cell cycle and its regulation, apoptosis, necrosis. 2. Reproduction of cells, mitosis and meiosis at the molecular level, consequences of these processes in carcinogenesis and reproduction process. 3. Process of carcinogenesis, molecular genetic methods and their application in pathology. Laser microdissection, Real Time PCR, chip technology. 4. Gene imprinting, its significance, examples of human genetics, methods of study. 5. Characterization of viruses, methods of their detection and characterization. 6. Differentiation and development of multicellular organism, problem of homeobox genes. 7. Immune system molecules, their function and importance in immune responses, conditionality of their diversity. 8. Methodological basics of biotechnology, cloning, vectors, restriction enzymes and their significance, methods of gene expression monitoring, transgenic animals. 9. Cloning of animals. 10. Gene therapy and possibilities of tissue engineering, pharmacogenetics. 11. Identification of persons, microchimerism, problems of free circulating nucleic acids. 12. Overview of methods studied - summary (methods of molecular genetics: PCR and its modification, sequencing, methods of gene expression analysis 13. cloning, overview of microscopic techniques, immunohistochemistry, in situ hybridization, event. visit a specialized laboratory.
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Learning activities and teaching methods
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Lecture with visual aids
- Contact hours
- 26 hours per semester
- Preparation for an examination (30-60)
- 30 hours per semester
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| prerequisite |
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| Knowledge |
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| to know the basic study of molecular and cell biology at the level of bachelor study of biology to know the basics of replication, transcription, translation and their regulation Know the types of distribution and cause of mutations to know the basics of DNA transposition to know the basic methods of molecular biology to know the basic science of genetics at the level of bachelor study of biology |
| Skills |
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| use the knowledge of a bachelor's degree program, or supplement the curriculum at this level analytically linking facts into a meaningful whole |
| Competences |
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| N/A |
| N/A |
| learning outcomes |
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| Knowledge |
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| Know the chemical composition of the cell, biopolymers, chaperons, genomics . |
| To know the genome and its structure. |
| To know the methods of studying genomes . |
| To know repetitive sequences and transposons. |
| To know the cytoskeletal principle of the cell organization, the components of the cytoskeleton and their function. |
| Know intercellular signaling, hormones and receptors. |
| Skills |
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| Describe the construction of prokaryotic and eukaryotic cells. |
| Use terminology of molecular biology. |
| Competences |
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| N/A |
| teaching methods |
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| Knowledge |
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| Lecture with visual aids |
| Skills |
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| Lecture |
| Competences |
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| Lecture |
| assessment methods |
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| Knowledge |
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| Written exam |
| Skills |
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| Written exam |
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Recommended literature
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Alberts, B. a kol. Základy buněčné biologie : úvod do molekulární biologie buňky. Ústí nad Labem: Espero Publishing, 1998. ISBN 80-902906-2-0.
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Bresler, Semen Jefimovič. Molekulární biologie. Praha : Academia, 1979.
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De Duve, Ch. A Guided Tour of the Living Cell. University Press, 1985.
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Karlson, Peter. Základy biochemie. Praha : Academia, 1981.
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Kubišta, V. Buněčné základy životních dějů. Praha, 1998.
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Kubišta, V. Obecná biologie. Fortuna Praha, 2000.
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Rosypal, S. a kol. Nový přehled biologie. Praha, 2003.
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Rosypal, Stanislav. Přehled biologie. Praha : Scientia, 1994. ISBN 80-85827-32-8.
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Rosypal, Stanislav. Úvod do molekulární biologie. 2.,rozš. vyd. Brno : Stanislav Rosypal, 1997.
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Šmarda J. Metody molekulární biologie. Masarykova univerzita, 2010. ISBN 9788021038417.
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Vlasák. Molekulární biologie.. Biologická fakulta JCU, 1998.
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Vodrážka, Zdeněk. Biochemie. Praha : Academia, 1996. ISBN 80-200-0600-1.
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Vonka, Z. a kol. Kapitoly molekulové biologie. H&H Praha, 1992.
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Žurovec, J. Molekulární biologie živočichů. Biologická fakulta JCU, 1999.
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