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Lecturer(s)
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Kout Jiří, Mgr. Ph.D.
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Houdek Zbyněk, Mgr. Ph.D.
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Course content
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Three days laboratory course. Safety work in laboratory. Sterilization, principes of aseptic work. Isolation of pure culture and work with the pure culture. Cultivation of bacteria. Native observation. Undifferencial and differencial staining of bacteria and their spores. Quantification of number of live cells. Methods of testing of antibiotic and chemoterapeutic effectivy.
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Learning activities and teaching methods
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Laboratory work
- Practical training (number of hours)
- 24 hours per semester
- Preparation for laboratory testing; outcome analysis (1-8)
- 8 hours per semester
- Preparation for comprehensive test (10-40)
- 20 hours per semester
- Preparation for an examination (30-60)
- 30 hours per semester
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| prerequisite |
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| Knowledge |
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| know basic of molecular biology and cytology at the bachelor study of biology level |
| know cell structure at the bachelor study of biology level |
| Skills |
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| be able to plan, carry out and conclude results from experiment according to task instruction |
| be able to cooperate fulfilling task with other students |
| be able to interpret results of observation using knowledge obtained during former study |
| Competences |
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| N/A |
| N/A |
| learning outcomes |
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| Knowledge |
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| adopt basic principes of laboratory work with microorganisms, their culativation in growth media and staining of microscopical preparates |
| Skills |
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| be able to verify theoretical information using planned experiment |
| hypothesize experimental results in concrete situation |
| Competences |
|---|
| N/A |
| N/A |
| teaching methods |
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| Knowledge |
|---|
| Laboratory work |
| Skills |
|---|
| Laboratory work |
| Competences |
|---|
| Laboratory work |
| assessment methods |
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| Knowledge |
|---|
| Skills demonstration during practicum |
| Skills |
|---|
| Skills demonstration during practicum |
| Competences |
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| Skills demonstration during practicum |
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Recommended literature
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Lékařská mikrobiologie. Praha : Marvil, 1996.
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Betina, V. a kol. Všeobecná mikrobiológia. Alfa Bratislava, 1977.
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Kaprálek, F. Mikrobiologické praktikum. Karolinum Praha, 1999.
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Rosypal, S. a kol. Obecná mikrobiologie. SPN Praha, 1981.
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