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Lecturer(s)
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Turková Sedláčková Terezie, Ing. Ph.D.
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Windrichová Jindra, RNDr. Ph.D.
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Course content
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Lectures: 1.General procedures in analytic chemistry. Harvest and preparation of samples for analysis. Preanalytic errors in laboratory medicine. 2.Types of analytic reactions. Quality parameters of analytical reactions. Selectivity and sensitivity of analytical reactions, imprecision and inaccuracy, reproducibility. 3.Analytic chemistry - qualitative, separation of inorganic ions into classes. Evidence of selected ions. 4.Basics of volumetric analysis: acidimetry, alcalimetry; Gravimetric analysis. 5.Comprehensive reaction and its application in laboratory analysis and volumetric methods; Precipitation reactions and their application in analytical chemistry. 6.Oxidation-reduction reaction and its application in a laboratory analysis. 7.Potentiometry, pH, ion selective electrode. 8. Principles of an organic analysis. 9.Spectrophotometry, flame photometry and atomic absorption spectrometry, infrared spectrometry. 10.Separation methods: Electrophoretic methods, chromatographic methods. 11.Mass spectrometry. 12.Immunochemical methods - immunoprecipitation, immunoanalysis. 13.Multiplex, chip technology, modern analytical approaches. Seminars: 1.-2. Patient´s preparation for harvest. Harvest systems. 3.-4. Calculations of parameters of quality in laboratory investigation. Preparation of control system in laboratory investigation. 5.-6. Application of flow cytometry (Multiplex,..). 7.-10.Laboratory practice - evidence of ions, volumetric determination. 11.-12. Laboratory practice - spectrum methods. 13. Credit test.
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Learning activities and teaching methods
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Lecture, Practicum
- Contact hours
- 39 hours per semester
- Preparation for comprehensive test (10-40)
- 5 hours per semester
- Preparation for an examination (30-60)
- 15 hours per semester
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| prerequisite |
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| Knowledge |
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| No particular prerequisites specified. |
| learning outcomes |
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| characterizes general procedures in the analysis of biological material |
| Skills |
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| possesses basic laboratory habits in the analytical field |
| calculates parameters of laboratory examination quality and monitors them |
| Competences |
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| N/A |
| teaching methods |
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| Knowledge |
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| Lecture |
| Skills |
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| Laboratory work |
| Competences |
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| Lecture |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Combined exam |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Combined exam |
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Recommended literature
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CROUCH, Stanley R., WEST, Donald M., HOLLER, F. James, SKOOG, Douglas A. Analytická chemie.. Praha: Vysoká škola chemicko-technologická v Praze, 2021. ISBN 978-80-7592-043-0.
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KLOUDA, Pavel. Moderní analytické metody. 3. vydání, upravené.. Ostrava: Pavel Klouda, 2019. ISBN 978-80-86369-22-8.
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ZÁRUBA, Kamil. Analytická chemie.. Praha: Vysoká škola chemicko-technologická v Praze, 2016. ISBN 978-80-7080-950-1.
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