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Lecturer(s)
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Hloušková Patricie, RNDr. Ph.D.
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Vaňous Jan, Ing.
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Hloušková Patricie, RNDr. Ph.D.
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Course content
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A Lab exercise 1. Introduction to work in a biochemical laboratory. OSH, PO 2. Reaction of basic functional groups of organic compounds. 3. Water and pH. Buffers, buffering capacity. pH measurement. 4. Properties of amino acids - determination, concentration, TLC 5. Determination of protein concentration - Biuret reaction, Lowry's method. Properties of protein solutions - isoelectric point, denaturation. 6. Separation of low-molecular and high-molecular substances - dialysis, gel chromatography. 7. Measurement of enzyme activity - effect of temperature, activity, specific activity. 8. Determination of basic kinetic parameters of enzyme reactions - Michaelis constant, maximum reaction speed. 9. Inhibition of enzyme activity - determination of inhibitory concentration IC50, influence of inorganic ions. 10. Myoglobin and hemoglobin - properties, determination, spectrophotometry. 11. Cholesterol and bile pigments. 12. Properties and determination of sugars, optical properties. 13. Properties of lipids. Biochemical examination of hyperlipoproteinemia. Lipoproteins (clinic). 14. Properties of nucleic acids. Isolation of DNA. Determination of DNA concentration and purity. 15. Credit test.
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Learning activities and teaching methods
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- Practical training (number of hours)
- 45 hours per semester
- Preparation for laboratory testing; outcome analysis (1-8)
- 5 hours per semester
- Preparation for comprehensive test (10-40)
- 25 hours per semester
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| prerequisite |
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| Knowledge |
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| to describe the basic components of biochemical systems |
| Skills |
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| to determine biologically significant ions and basic organic structures |
| Competences |
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| N/A |
| learning outcomes |
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| Knowledge |
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| describes the structure and characterizes the reactions of individual molecules |
| defines the possibilities of determining specific substances |
| describes the principle of the basic analytical methods of spectrophotometry, electrophoresis, chromatography and pH measurement |
| Skills |
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| prepares and measures solutions |
| works with an automatic analyzer |
| performs biochemical tests independently |
| Competences |
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| N/A |
| teaching methods |
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| Knowledge |
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| Laboratory work |
| Skills |
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| Laboratory work |
| Competences |
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| Laboratory work |
| assessment methods |
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| Knowledge |
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| Test |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Test |
| Skills demonstration during practicum |
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Recommended literature
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KODÍČEK, Milan, Olga VALENTOVÁ a Radovan HYNEK. Biochemie: chemický pohled na biologický svět. 3. přepracované vydání.. Praha: Vysoká škola chemicko-technologická v Praze, 2022. ISBN 978-80-7592-124-6.
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