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Lecturer(s)
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Karlíková Marie, RNDr. Ph.D.
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Course content
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Lectures 1. - 2. Use and specifics of application of immunoanalytical methods in cardiology and gastroenterology of metabolic diseases. 3. - 4. Use of immunoanalytical methods in oncology. 5. - 6. Use of immunoanalysis for screening programs. 7. - 8. Immunoanalysis and proteomics and the use of immunoanalysis in personalized medicine. 9. - 10. Immunochemical methods - immunoprecipitation, immunoanalysis. A Lab exercise 1. - 2. Determination of the selected biomarker by the ELISA method. Evaluation of measurement results from an analytical point of view. 2. - 3. Evaluation of immunoassay quality parameters - accuracy, correctness, sensitivity, measurement result, working interval. 4. - 5. Measurement uncertainty, measurement errors. Internal and external quality control. Evaluation of the result in terms of contribution to the diagnosis. 6. - 7. Interpretation of results for endocrinology. 8. - 9. Interpretation of the results of tumor markers. 10. Credit test.
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Learning activities and teaching methods
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Lecture, Practicum
- Contact hours
- 20 hours per semester
- Preparation for comprehensive test (10-40)
- 8 hours per semester
- Preparation for laboratory testing; outcome analysis (1-8)
- 2 hours per semester
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| prerequisite |
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| Knowledge |
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| to describe the body's immune response based on the production of antibodies |
| Skills |
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| controls the principle of ELISA immunochemical methodology using an enzyme to mark an antigen or an antibody |
| Competences |
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| N/A |
| N/A |
| learning outcomes |
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| Knowledge |
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| will describe highly specific and highly sensitive (nmol - pmol) procedures in the analysis of biological material |
| Skills |
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| has basic laboratory habits in the field of immunoanalysis |
| works with selected laboratory instruments |
| evaluates examination quality parameters |
| Competences |
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| N/A |
| N/A |
| teaching methods |
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| Knowledge |
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| Lecture with visual aids |
| Lecture |
| Practicum |
| Skills |
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| Laboratory work |
| Skills demonstration |
| Competences |
|---|
| Lecture with visual aids |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Test |
| Skills |
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| Skills demonstration during practicum |
| Continuous assessment |
| Competences |
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| Skills demonstration during practicum |
| Continuous assessment |
| Test |
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Recommended literature
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BARTOŠ, Vladimír, ŠAFARČÍK, Kristián, KARLÍKOVÁ, Marie, TOPOLČAN, Ondřej, KUČERA, Radek a Jindra WINDRICHOVÁ. Principy imunoanalytických metod pro mediky.. 2013.
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Štern P., Kocna P. Obecná a klinická biochemie pro bakalářské obory studia,Karolinum, Praha 2004.
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Wild D. (Ed.). The Immunoassay Handbook Theory and Applications of Ligand Binding, ELISA and Related Techniques. Fourth Edition. Elsevier, 2013. ISBN 978-0-08-097037-0.
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ZIMA, Tomáš. Laboratorní diagnostika. Třetí, doplněné a přepracované vydání.. Praha : Galén, 2013. ISBN 978-80-7492-062-2.
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