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Lecturer(s)
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Vimmerová Hana, Mgr.
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Kukrálová Simona, Mgr.
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Cibulka Roman, MUDr. Ph.D., MBA
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Brož Pavel, MUDr. Ph.D.
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Bršlicová Kateřina, Ing.
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Prokop Pavel, RNDr. DiS.
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Course content
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Lectures 1. Clinical biochemistry and its position among laboratory disciplines. Organization of clinical biochemistry services in the Czech Republic. 2. - 3. Preanalytical influences on the results of laboratory examination. 4. - 6. Analytical properties of the laboratory method. Accuracy, truthfulness, analytical sensitivity and specificity. Quality control of laboratory examination. Features of the laboratory method from the clinical point of view. Diagnostic sensitivity (sensitivity) and specificity, the relationship between diagnostic sensitivity and specificity, some other indicators of the clinical evaluation of a laboratory test. Reference values of laboratory examinations. Definition, basic terms, methods of determining the reference range, method of comparing the measured value with reference values. 7. - 9. Internal environment. Metabolism of water, sodium, potassium and chlorides. Osmolality. Metabolism of calcium, magnesium and phosphorus. Acid-base balance and its disorders, blood gases. 10. - 11. Basic functions of the kidneys and their disorders, glomerular filtration, tubular function. Non-protein nitrogenous substances - urea, creatinine, uric acid, ammonia, amino acids. Replacement of kidney function and the role of the laboratory in it. 12. Blood plasma proteins. Meaning of plasma proteins, individual proteins of blood plasma. Immunochemical methods: basic concepts, reactions in gels, immunoturbidimetry and -nephelometry, methods using a label. 13. Cardiovascular apparatus. Risk factors for the development of atherosclerosis. Laboratory diagnosis of myocardial infarction and heart failure. A Lab exercise 1. - 2. Basic calculations in the clinical laboratory. Concentration of solutions, pH, buffers. 3. - 4. Qualitative and quantitative examination of urine. 5. - 6. Disorders of glucose metabolism, diabetes mellitus. 7. - 8. Minerals and osmolality in serum and urine, trace elements. 9. - 10. Proteins in serum. 11. - 12. Lipids and lipoproteins in serum, disorders of lipid metabolism, atherosclerosis and its complications. 13. Credit test
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Learning activities and teaching methods
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- Contact hours
- 91 hours per semester
- Preparation for laboratory testing; outcome analysis (1-8)
- 8 hours per semester
- Preparation for comprehensive test (10-40)
- 30 hours per semester
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| prerequisite |
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| Knowledge |
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| to characterize the general principles of metabolism |
| Skills |
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| to build on knowledge from biochemistry |
| Competences |
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| N/A |
| N/A |
| learning outcomes |
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| Knowledge |
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| demonstrates broad knowledge of changes in the chemical composition of the organism that can be used to determine the diagnosis, prognosis and follow the course of the disease |
| will explain the importance of reference values and decision limits, the importance of analytical and clinical properties of laboratory methods and their use in laboratory medicine |
| defines the difference between laboratory test reference values and decision limits |
| Skills |
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| distinguishes between diagnostic sensitivity and specificity |
| compares measured values with reference values |
| performs basic calculations in the clinical laboratory (pH, buffers, concentration of solutions) |
| performs qualitative and quantitative urine tests and serological tests |
| Competences |
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| N/A |
| N/A |
| teaching methods |
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| Knowledge |
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| Lecture |
| Skills |
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| Laboratory work |
| Competences |
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| Lecture |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Test |
| Skills |
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| Skills demonstration during practicum |
| Continuous assessment |
| Competences |
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| Skills demonstration during practicum |
| Continuous assessment |
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Recommended literature
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CIBULKA, Roman. Kontrola kvality laboratorního vyšetření..
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CIBULKA, Roman. Laboratorní diagnostika infarktu myokardu..
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CIBULKA, Roman. Rizikové faktory aterosklerózy..
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DASTYCH, Milan, BREINEK, Petr a kol. Klinická biochemie: bakalářský obor Zdravotní laborant. 3. vydání, 1. dotisk. Brno: Masarykova univerzita, 2021. ISBN 978-80-210-7788-1.
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RACEK, Jaroslav. Preanalytické vlivy na laboratorní vyšetření..
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RACEK, Jaroslav, RAJDL, Daniel et al. Klinická biochemie. Třetí, přepracované a rozšířené vydání.. Praha : Galén, 2021. ISBN 978-80-7492-545-0.
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RACEK, Jaroslav. Referenční hodnoty..
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RACEK, Jaroslav. Vyšetření moči..
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ZIMA, Tomáš. Laboratorní diagnostika. Třetí, doplněné a přepracované vydání.. Praha: Galén, 2013. ISBN 978-80-7492-062-2.
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