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Lecturer(s)
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Fajfrlík Karel, RNDr. Ph.D.
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Fajfrlík Karel, RNDr. Ph.D.
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Course content
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Lectures 1. Introduction to the subject. An overview of the history of microbiology. Principles of laboratory animal care. 2. Pathogenic mechanisms, infection process, virulence factors. 3. Morphology of the bacterial cell. 4. Use of antibiotics and chemotherapeutic agents in microbiology. Antimicrobial preparations. 5. Bacterial taxonomy - explanation of the concept. The importance of bacteria and viruses in the development of diseases. 6. Infectious diseases - prevention and prophylaxis. 7. - 8. Anti-infective immunity - clinical implications in the treatment of infectious diseases. 9. Microflora in the human body - meaning, localization. 10. Virology - prions. 11. Mycology - basic knowledge. 12. Parasitology - basic knowledge. 13. Sepsis from the point of view of microbiology. 14. Perinatal infections from the point of view of microbiology. 15. Resistance of microorganisms. A Lab exercise 1. Principles of occupational safety and health protection when working in a microbiological laboratory. Occupational and laboratory infections. 2. Sterilization and disinfection in microbiology. 3. Microbiological laboratory equipment. 4. Reproduction in laboratory conditions - cultivation soils, tissue cultures, chicken embryos, experimental animals. 5. Collection and transport of biological samples to the microbiological laboratory. 6. Methods of species identification of microorganisms - light microscopy, cultivation, biochemical, immunological and molecular methods. 7. Theoretical foundations of staining and microscopic examination. 8. Gram+, gram- cocci, gram- rods. 9. Gram+ rods, anaerobic bacteria. 10. Acid-resistant rods, spirochetes. 11. Mycoplasma, chlamydia, rickettsia. 12. Sexually transmitted infections. 13. DNA viruses. 14. RNA viruses. 15. Credit test.
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Learning activities and teaching methods
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Lecture
- Contact hours
- 60 hours per semester
- Preparation for comprehensive test (10-40)
- 20 hours per semester
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| prerequisite |
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| Knowledge |
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| present basic knowledge of the biology of living organisms |
| Skills |
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| specific skill prerequisites are not required |
| Competences |
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| N/A |
| learning outcomes |
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| Knowledge |
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| at a basic level, he will describe the morphology of microorganisms and explain their pathogenic effect on humans, |
| characterizes the physiology of bacterial cultures and explains the metabolism of microorganisms |
| describes the effects of antibiotics on microorganisms |
| lists the principles of prevention and prophylaxis of infectious diseases |
| will describe the types of staining techniques in the microbiology laboratory |
| Skills |
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| masters the basics of bacterial taxonomy |
| collects and transports the biological sample to the microbiology laboratory |
| evaluates violations of safety principles when working in a microbiological laboratory |
| observes the principles of health protection when handling the biological sample |
| evaluates the method of reproduction in laboratory conditions using culture media, tissue cultures, chicken embryos or experimental animals |
| determines the method of species identification of microorganisms (light microscopy, cultivation, biochemical, immunological and molecular methods). |
| Competences |
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| N/A |
| teaching methods |
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| Knowledge |
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| Lecture |
| Multimedia supported teaching |
| Skills |
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| Laboratory work |
| Competences |
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| Lecture |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Test |
| Continuous assessment |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Skills demonstration during practicum |
| Test |
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Recommended literature
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DRNKOVÁ, Barbora. Mikrobiologie, imunologie, epidemiologie a hygiena pro zdravotnické obory.. Praha : Grada, 2019. ISBN 978-80-271-0693-6.
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GOERING, Richard V., DOCKRELL, Hazel M., ZUCKERMAN, Mark, ROITT, Ivan M. a Peter L. CHIODINI. Mimsova lékařská mikrobiologie. 5. vydání. Editor Jaroslav JULÁK.. Praha: Stanislav Juhaňák - Triton, 2016. ISBN 978-80-7387-928-0.
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HURYCH, Jakub, ŠTÍCHA, Roman et al. Lékařská mikrobiologie - repetitorium.. Praha: Triton, 2021. ISBN 978-80-7553-976-2.
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KOLÁŘOVÁ, Libuše et al. Obecná a klinická mikrobiologie.. Praha : Galén, 2020. ISBN 978-80-7492-477-4.
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MELICHERČÍKOVÁ, Věra. Sterilizace a dezinfekce. Druhé, doplněné a přepracované vydání.. Praha: Galén, 2015. ISBN 978-80-7492-139-1.
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VOTAVA, Miroslav a kol. Lékařská mikrobiologie - vyšetřovací metody.. Brno: Neptun, 2010. ISBN 978-80-86850-04-7.
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