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Lecturer(s)
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Cibulka Roman, MUDr. Ph.D., MBA
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Bršlicová Kateřina, Ing.
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Course content
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Content of the professional practice: HISTOLOGY - 2 weeks - Preparation of material for histologic use. - Elemental freezing technique. - Technique of elaboration of cutting samples on mikrotome. - Hematoxylin - eosin dyeing. - Mallory's trichrome. - Histochemistry of anorganic substances. - Feulgen reaction, methyl green, apyronin. - PAS, Best's carmine. - Staining of mucopolysaccharides, mucoproteins and glycoproteins. - Sudanophilia, OsO4. - Enzyme staining. - Epithelial tissue, connective tissue, muscular tissue, nervous tissue. - Circulatory system, respiratory system, lymphatic system, endocrine system, gastrointestinal tract, urinary tract, reproductive system, integumentary system, nervous system, senses. - Evaluation of practical professional training. IMMUNOLOGY - 2 weeks - Internal and external control of investigation quality in immunologic laboratory - Material harvesting. - ELISA, RIA. - Immunofluorescent methods, IgA, IgE. - CRP. - Antibodies for grasses. - Antibodies for milk and gliacine. - Antibodies for mites. - Complement. - Immunocomplexes. - Setting of T-lymphocytes. - Opsonization test. - NBT test. - Evaluation of practical professional training.
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Learning activities and teaching methods
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Clinical practice under supervision
- Practical training (number of hours)
- 160 hours per semester
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| prerequisite |
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| Knowledge |
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| No particular prerequisites specified. |
| learning outcomes |
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| A student will be able to harvest biological material, process, cut, stain. He/she will be able to determine the antibodies at immunological level. |
| teaching methods |
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| Clinical practice under supervision |
| assessment methods |
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| Skills demonstration during practicum |
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Recommended literature
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Bartůňková, J., Paulík, M. a kol. Vyšetřovací metody v imunologii. Praha Grada Publishing, 2005. ISBN 80-247-0691-1.
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Horký, D., Čech, S. Základy histologické techniky pro zdravotní laboranty. Skripta LFMU. MU Brno, 2007. ISBN 13: 978-80-210-44.
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Hořejší, V., Bartůňková, J. Základy imunologie. 3. vydání. Praha Triton, 2009. ISBN 80-254-686-4.
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Junqueira, L.C., Carneiro, J., Kelley ,R.O. Základy histologie. Jinočany: H&H, 2002. ISBN : 8085787377.
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Konrádová, V., Uhlík, J., Vajner, L. Funkční histologie. Martinus, 2000. ISBN 80-8602-280-3.
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