Course: Professional training in clinical laboratory 4 - clinical biochemistry, transfusion service, clinical genetics

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Course title Professional training in clinical laboratory 4 - clinical biochemistry, transfusion service, clinical genetics
Course code KAZ/OP4N
Organizational form of instruction Seminar
Level of course Bachelor
Year of study not specified
Semester Summer
Number of ECTS credits 13
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Cibulka Roman, MUDr. Ph.D., MBA
Course content
CLINICAL BIOCHEMISTRY - 5 weeks - Internal and external quality control of investigation, record keeping and result distribution, organisation of operation. - Preparatory and auxiliary work - disinfection, sterilization, cleaning of laboratory tools. - Urine investigation - harvesting systems, quantita, colour, odour, turbidity, foam, biochemic composition (proteins, glycides, ketone bodies, biliary stain, bilirubin, urobilinogen, urobilin, hemoglobin, phenylpyruvic acid, urinary sediment - blood cells, epithelium, cylinders, crystals.) - Preanalytic phase control. - Faeces investigation. - Setting of serum proteins. - Urea, creatinine, creatine, uric acid. - Aminoacids in serum. - Selected immunochemical investigation. - ABR investigation. - Toxicologic investigation. - Evaluation of training. TRANSFUSION SERVICE - 2 weeks - Record keeping and distribution of the results, organisation of operation, SMJ, SVP and manipulation with LIS. - Laboratory investigation of blood donors. - Laboratory proof of blood types and blood compatibility. - Investigation of antibodies. - Investigation of leukocytary and thrombocytary antigenes. - Investigations to reduce the risk of transmission of infectious diseases by transfusion tools. - Evaluation of the training. - Immunohematologic investigation in hemolytic disease of the new-born. MEDICAL GENETICS - 2 weeks - Internal and external quality control in a genetic laboratory. - Organisation of operation, record keeping and distribution of results. - Methods of genetic analyses, heredity types assessment. - Tissue cultures in vitro and in situ, construction and structure of chromosomes. - Striping methods, fluorescence in situ hybridization, modern methods of chromosomal analyses (FISH, CGH). - Cytogenetic methods - PRINS, mFISH, mBAND FISH, SKY, CGH, array CGH. - Analysis of chromosomal aberrations. - Harvesting of biological material. - DNA isolation - desalination method. - PCR, electrophoresis, restrictive cleavage, real-time PCR. - Testing of genotoxicity. - Evaluation of training. INVESTIGATIVE METHODS IN PUBLIC HEALTH PROTECTION - 2 weeks - according to the specialization of a particular workplace: - Physical measurement for public health protection - noise, vibration, light, electromagnetic radiation. - Volumetric, gravimetric and instrumental analysis of food, water, waste and air. - Biological exposure tests.

Learning activities and teaching methods
Clinical practice under supervision
  • Contact hours - 440 hours per semester
prerequisite
Knowledge
No particular prerequisites specified.
learning outcomes
A student will be able to perform individually routine clinical biochemic investigation, investigation of blood types, investigation of blood donors, antibodies, antigenes, investigation of hemolytic disease of a new-born. Will be able to do the basic genetic investigations (describe the construction and stucture of chromosomes, types of heridity, DNA isolation, genotoxic investigation procedures).
teaching methods
Clinical practice under supervision
assessment methods
Individual presentation at a seminar
Recommended literature
  • Sbírka příkladů pro praktická cvičení. Základní studijní literatura pro studenty 1. LF UK..
  • Věstník MZ 2003, částka 8 Řešení problematiky infekce HIV/AID.
  • Vyhláška 143/2008 o stanovení bližších požadavků pro zajištění jakosti a bezpečnosti lidské krve a jejích složek (vyhláška o lidské krvi).
  • Zákon 378/2007 Sb. - Zákon o léčivech.
  • Dastych, M., Hrdinek, P. a kol. Klinická biochemie, bakalářský obor Zdravotní laborant. Masarykova Univerzita, Lékařská fakulta, Brno, 2008.
  • Hrubiško, M. a kol:. Hematologie a krevní transfuze II - Krevní transfuze.. Avicenum, Praha, 1983.
  • Hůnová, Iva; Janoušková, Svatava. Úvod do problematiky znečištění venkovního ovzduší. 1. vyd. Praha : Karolinum, 2004. ISBN 80-246-0796-4.
  • Kapras, J.,Kohoutová, M. Kapitoly z lékařské biologie a genetiky III. 1. vydání. Praha : Karolinum, 1999. 101 s.. ISBN 80-246-0001-3.
  • Kapras, J.,Kohoutová, M., Otová, B. Kapitoly z lékařské biologie a genetiky I. 1. vydání. Praha : Karolinum, 1996. 88 s..
  • Kočárek, E.:. Genetika: obecná genetika a cytogenetika, molekulární biologie, biotechnologie, genomika. 1. vydání. Praha, Scientia, 2004. 211 s.. ISBN 80-7183-326-6.
  • Kočárek, E., Pánek, M., Novotná, D. Klinická cytogenetika I.: úvod do klinické cytogenetiky, vyšetřovací metody v klinické cytogenetice. 1. vydání. Praha : Karolinum, 2006. 120 s.. ISBN 80-246-1069-8.
  • Kotlas, J., et al. Návody a úkoly k praktickým cvičením z lékařské biologie a genetiky. 1. vydání. Praha : Karolinum, 2000. 148 s.. ISBN 80-246-0065-X.
  • Otová, B., et al. Lékařská biologie a genetika I. díl. 1. vydání. Praha : Karolinum, 2008. 123 s.. ISBN 978-80-246-1594-3.
  • Pritchard, Dorian J. - Korf, Bruce R. Základy lékařské genetiky. 1. vydání.. Galén, Praha, 2007. ISBN 978-80-7262-449-2.
  • Sakalová, A. a kol. Klinická hematológia. Osveta, 2010. ISBN 978-80-8063-324-0.
  • Soukupová, M., Soukup, F. Kapitoly z lékařské biologie a genetiky II. 1. vydání. Praha : Karolinum, 1998. 98 s.. ISBN 80-7184-581-7.
  • Štern, P. a kol. Obecná a klinická biochemie pro bakalářské obory studia - CD ROM. Karolinum, Praha, 2005.
  • Thompson, James Scott - Thompson, Margaret Wilson - Nussbaum, Robert L, et al. Klinická genetika: Thompson & Thompson. 6. vydání. Praha : Triton, 2004. 426 s.. ISBN 80-7254-475-6.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester