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Lecturer(s)
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Course content
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MICROBIOLOGY - 2 weeks (80 hours) Content content of practice - operating rules of the relevant microbiological laboratory, principles of work safety, principles of disinfection and sterilization. - receipt and identification of material, SOP - preparation of cultivation media and possibly sterility verification - examination of the throat, nose, swabs from the ear, conjunctival sac - examination of wounds - examination of sputum, bronchial lavage of pus - examination of urine, urethral swabs - stool examination - examination of dialysate, punctate - cerebrospinal fluid examination - examination of cannula, catheter - blood examination from blood culture - anaerobic examination - mycological examination - examination of part of the tissue - examination for Helicobacter pylori from a biopsy sample - examination of the vaginal image - examination of mycoplasma, ureaplasma - Chlamydia examination - sterility examination - determining the minimum inhibitory concentration of the antibiotic and allowing bound antibiotics - examination of Legionella pneumophila HISTOPATHOLOGY - 2 weeks (80 hours) Content content of practice - preparation of material for histological use - basic freezing technique - the technique of making microtome sections - hematoxylin - eosin staining - Mallory staining - histochemistry of inorganic substances - Feulgen reaction, methyl green, apyronin - PAS, Best's crimson - staining of mucopolysaccharides, mucoproteins and glycoproteins - sudanophilia, OsO4 - enzyme staining - epithelial tissue, connective tissue, muscle tissue, nervous system, circulatory system, respiratory system, lymphatic system, glands with internal secretion, digestive system, urinary system, sexual system, skin, and nervous system, senses IMMUNOLOGY - 2 weeks (80 hours) Content content of practice - internal and external quality control of examinations in the immunological laboratory, sampling of material - ELISA, RIA - immunofluorescence methods, IgA, IgE - CRP - antibodies against grasses - antibodies against milk and gliacin - antibodies against mites - complement - immunocomplexes - determination of T lymphocytes - opsonization test - NBT test
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Learning activities and teaching methods
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- Practical training (number of hours)
- 240 hours per semester
- Presentation preparation (report) (1-10)
- 5 hours per semester
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| prerequisite |
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| Knowledge |
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| to describe the microanatomical structure of tissues, organs and organ systems |
| to describe methods of collecting histological material and explain the technique of processing a sample of tissue or cells for microscopic analysis |
| to explain the procedure for processing hard tissues for histological examination |
| to characterize individual types of staining |
| to describe the types of staining techniques in the microbiology laboratory |
| to explain the principles of laboratory methods for monitoring humoral and cellular immunity |
| to explain the principle of ELISA immunochemical methodology using an enzyme to mark an antigen or an antibody |
| Skills |
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| to classify individual tissues |
| independently to perform fixation, embedding, staining and sectioning of tissues |
| to collect and transport a biological sample to the microbiology laboratory |
| to observe health protection principles when handling a biological sample |
| to determine the method of species identification of microorganisms (light microscopy, cultivation, biochemical, immunological and molecular methods) |
| to perform protein detection under supervision, including Ig nephelometry and turbidimetry and detection of circulating immunocomplexes |
| by the flow cytometry method to determine under supervision the phenotyping of T and B lymphocytes, the basophil activation test, blast transformation of lymphocytes and oxidative burst |
| Competences |
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| N/A |
| N/A |
| N/A |
| learning outcomes |
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| Knowledge |
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| defines the content of the laboratory manual, operating and hygiene regulations of the microbiological, histopathological and immunological laboratory |
| Skills |
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| applies the principles of aseptic work in the microbiology laboratory in order to prevent contamination of samples with live microbial cells during laboratory processing |
| evaluates the external and internal quality of the microbiological examination of the collected biological material |
| processes the collected sample from a technical and methodological point of view and describes it |
| determines sensitivity to ATB |
| prepares biological material for histological use, processes it, cuts and stains it |
| masteres basic freezing technique and work on a microtome |
| determines antibodies, number and differential budget of leukocytes in peripheral blood |
| works with a flow cytometer, turbidimeter or nephelometer and controls instrumentation for performing and evaluating ELISA reactions |
| prepares preparations for immunofluorescence examination |
| Competences |
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| N/A |
| N/A |
| N/A |
| teaching methods |
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| Knowledge |
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| Clinical practice under supervision |
| Skills |
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| Clinical practice under supervision |
| Competences |
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| Clinical practice under supervision |
| assessment methods |
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| Knowledge |
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| Continuous assessment |
| Skills |
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| Skills demonstration during practicum |
| Individual presentation at a seminar |
| Competences |
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| Skills demonstration during practicum |
| Continuous assessment |
| Individual presentation at a seminar |
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Recommended literature
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BARTŮŇKOVÁ, Jiřina, PAULÍK, Milan a kol. Vyšetřovací metody v imunologii. 2., přepracované a doplněné vydání.. Praha : Grada, 2011. ISBN 978-80-247-3533-7.
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JIRKOVSKÁ, Marie. Histologická technika: základy. Druhé, doplněné vydání.. Praha : Galén, 2017. ISBN 978-80-7492-325-8.
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VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ a Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody. 1. vyd.. Brno: Neptun, 2010. ISBN 978-80-86850-04-7.
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VOTAVA, Miroslav. Lékařská mikrobiologie II.: přehled vyšetřovacích metod v lékařské mikrobiologii.. Brno: Masarykova univerzita, 2000. ISBN 80-210-2272-8.
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