Lectures 1. Toxicology. Introduction, history, subfields (clinical, industrial, experimental, etc.), sources and applications of toxicology. 2. Poisons. Definition, classification, labeling, dose-effect, toxicokinetics (entry, biotransformation, excretion). 3. Types of materials. Sampling, treatment before analysis (isolation, extraction, separation, mineralization, etc.). 4. Toxicological examinations. Reasons, known - unknown exposure, screening, biological exposure tests - BET, choice investigated substances according to time since exposure, interpretation (highest permissible limits, NPK). 5. Analytical techniques. Principles of quantitative methods (spectrophotometric, chromatographic, immunochemical, etc.). 6. Poisoning by substances blocking the transfer of oxygen. 7. Alcohol poisoning (ethanol, methanol, ethylene glycol). 8. Poisoning by heavy metals, solvents, "-icides" (herbicides, pesticides, fungicides), rarer poisonings. 9. Poisoning by substances from the living and domestic environment (plants, animals, drug preparations, medicines). 10. Drug level monitoring (TDM), pharmacokinetic evaluation. 11. Drugs and Substances of Abuse. Classification of groups and effects, screening, classic drugs - new drugs. 12. Clinical manifestations of intoxication. General principles of therapy. A Lab exercise 1. Principles of safety in the toxicological laboratory, devices and aids. 2. - 3. Biological material - types, quantity, method of preservation, processing time, documentation. 4. - 5. Treatment of samples, deproteinization, extraction, hydrolysis, isolation, mineralization. 6. Analytical methods in toxicology - immunochemical. 7. Analytical methods in toxicology - chromatographic (TLC, GC, HPLC). 8. Analytical methods in toxicology - spectrophotometric (AAS, UV-VIS, IC). 9. Organization of work in the toxicological laboratory, toxicological analysis in POCT mode. 10. Issues of forensic toxicology (collection and preservation of material, recognized methods, laboratory equipment). 11. Excursion to the Toxicology Laboratory of the Institute of Forensic Medicine of the Pilsen University Hospital. 12. Credit test.
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BALÍKOVÁ, Marie. Forenzní a klinická toxikologie: laboratorní toxikologická vyšetření. Druhé, doplněné vydání.. Praha : Galén, 2017. ISBN 978-80-7492-304-3.
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McGACHY, Lenka, TOMÁŠOVÁ , Pavla a Zuzana ROŠKOVÁ. Toxikologie a ekotoxikologie I. Praha: Vysoká škola chemicko-technologická v Praze,, 2021. ISBN 978-80-7592-097-3.
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RACEK, Jaroslav. Základy toxikologie. In RACEK, Jaroslav, RAJDL, Daniel et al. Klinická biochemie. Třetí, přepracované a rozšířené vydání.. Praha : Galén, 2021. ISBN 978-80-7492-545-0.
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