Lectures: A. Immunohaematology and HLA: 1. Familiarization with the professional field (concept and definition of transfusion services, blood transfusion history, basic concepts of immuno - antigens and antibodies in general). 2. Blood groups and their antibodies, complement, agglutination, hemolysis, precipitation. 3. Group systems of erythrocytes and antibodies - AB0H system, history of discovery, the importance, heredity, occurrence. 4. Subgroups and variants of AB0H system, properties of blood groups. Lewis group system. 5. Rh group system. 6. Other erythrocyte group systems (antigens, antibodies). 7. Significance of the blood groups properties - classification, significance for transfusion, immunogenetics and for hemolytic anemia (including AIHA). 8. Haemolytic disease of newborn (causes, clinical manifestations, treatment, prevention). 9. Antigens of thrombocytes and leukocytes. Practical importance. 10. HLA system (genes, antigens, antibodies, laboratory diagnostics). 11. HLA system - practical significance during the haemotherapy, in the pregnancy, in relation to diseases, for transplantation (organ transplantation, bone marrow and stem cell transplatation, for donor registries, for ensuring of the immunohaematological transplantation). B. Transfusion service 1. Organisation of blood transfusion services, legislation, proper practice in transfusion service facilities. Information systems in the blood transfusion services. 2. Blood donation - blood donors´ registers, blood donors´ assessment of eligibility, compulsory testing of blood donors, blood sampling and allied complications. 3. Blood stabilizers, preservative solutions, blood bags, production of blood products including special procedures (deleucotization, elution), standard in the blood products labeling. 4. Special sampling - separating techniques, autotransfusions (types of autologous donations, special working procedures with regard to donor - patient - blood.) Therapeutic aphereses, transplantation of stem cells, umbilical cord blood. 5. Blood products (specifications, conditions of storage, distribution, transportation) and blood derivatives. 6. After sampling examinations of donors, the release of blood products, control in the transfusion services (quality control, bacteriological control, internal quality control). 7. Prevention of the infectious diseases transmission via the blood products. 8. Clinical transfusion practice - effective haemotherapy - indication for blood transfusion. 9. Duties and job description in blood stocks (banks), transfusion committee, contact of blood storage with other departments, pretransfusion testing, universal donor, universal recipient. Administration of blood transfusion (preparatory work on the clinical department, administration technique). 10. Post-transfusion reactions and complications, haemovigilance. 11. Massive transfusion, transfusion during mass disasters, emergency blood transfusion centers. Laboratory practicals: 1. Familiarization with the workplace of Transfusion department. Principles of work in the immunohemathologic lab and work with biological material. Health and safety training. The diagnostic serum and type cells. 2. Complete examinations of blood groups in the AB0 system. Examination of subgroups in the AB0 system. Examination of the Rh D antigen, including D w / v. 3. Determining of antigens within the other of erythrocyte group systems. 4. Examination of anti-erythrocyte antibodies - screening, identification, assessment. 5. The direct antiglobulin test, refine sensitization erythrocytes. Specification of erythrocytes sensitization. 6. Quantitative examination of anti-erythrocyte antibodies - titration. 7. Examination of the universal donor.Compatibility test. 8. Examination for hemolytic disease of the newborn. Immune anti-A, anti-B. 9. Exsanguination 10. Examination of post-transfusion reaction. 11. HLA laboratory diagnostics
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Vyhláška 143/2008 o stanovení bližších požadavků pro zajištění jakosti a bezpečnosti lidské krve a jejích složek (vyhláška o lidské krvi) v platném znění.
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www.transfuznispolecnost.cz: legislativa, doporučené postupy, přednášky, semináře.
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Zákon 378/2007 Sb. - Zákon o léčivech.
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DOBROTOVÁ, M. Hematológia a transfuziológia. Praha:Grada, 2006. ISBN 80-247-1779-4.
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ECKSTEIN, R. Imunohematologie a transfuzní lékařství. Guide to the Preparation, Use and Quality Assurance of Blood Components, poslední vyd.
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PECKA, M. Základy imunohematologie. Hradec Králové: VOŠZ a SZŠ, Astra Print, 2005. ISBN ISBN 80-90341-4-6.
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PENKA, M. a TESAŘOVÁ E. Hematologie a transfuzní lékařstv II.. 1, vyd. Praha: Grada, 2012. ISBN 978-80- 247-3460-.
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PENKA, M., SLAVÍČKOVÁ, E. Hematologie a transfuzní lékařství. 1. vyd. Praha: Grada, 2011. ISBN 978-802-4734-590.
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Řeháček, Vít; Masopust, Jiří. Transfuzní lékařství. 1. vyd. Praha : Grada, 2013. ISBN 978-80-247-4534-3.
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TESAŘOVÁ E. Vybrané kapitoly z imunohematologie a transfuzní služby. Brno., 2007.
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