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Lecturer(s)
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Turková Sedláčková Terezie, Ing. Ph.D.
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Hejdová Jana, Ing.
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Fajfrlík Karel, RNDr. Ph.D.
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Course content
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Lectures 1. Chemical investigation methods of analysis of water processed in the hygiene laboratory - drinking, raw, manufactured drinking, warm, bathing (natural and artificial swimming pools, saunas, pools), waste, packaged (mineral, infant) - according to applicable legislation, purified according to the Czech Pharmacopoeia . 2. Chemical investigation methods for determining indicators during PBU investigations - products for contact with drinking water, products for contact with food, products for children under 3 years of age, cosmetic products - according to applicable legislation. 3. Investigation methods for the determination of pollutants in municipal air (indoor, outdoor) - chemical including sampling, automatic air analyzers - mobile and stationary sampling. 4. Chemical investigation methods for waste determination, including sample collection and preparation, waste leachability classes, ecotoxicological tests - according to applicable legislation. Investigative methods for determining indicators in sediments, soils, mud and sand, including sampling and preparation of samples - according to applicable legislation. 5. Chemical investigation methods for determining analytes in food - inorganic and organic indicators - according to valid legislation. 6. Chemical investigation methods for determination of gaseous pollutants in the working atmosphere, including sampling, sampling and determination of dust content, PEL - according to applicable legislation. 7. Detection and measurement of noise in the work and communal environment, measurement of overall vibrations in the work environment. 8. Electromagnetic field measurement, spectral analysis. Measurement of lighting - daytime, artificial, assessment of visual conditions, detection of glare. Determination of microclimatic conditions - temperature, humidity, flow speed. 9. Microbiological factors of the environment - types of samples, techniques of collection and transport of samples for microbiological examination 10. Microbiological methods in water, soil and waste hygiene. Microbiological investigation of foods. A Lab exercise 1. System of work in a hygiene laboratory. Quality and quality assurance - MPZ (interlaboratory comparative tests), authorization, accreditation. Safety and health protection when working in a hygienic laboratory. Management of laboratory documentation, standard operating procedures, protocols. 2. Examination of chemical organoleptic and health-related indicators of drinking water quality. Investigation of chemical indicators of bathing water quality and their sources. Leaching tests for materials and products coming into contact with drinking water. 3. Investigation of inorganic and organic contaminants in objects of common use and food packaging. Perfluorinated compounds in consumables. 4. Investigation of organic and inorganic pollutants in the air. Determination of polycyclic aromatic hydrocarbons. 5. Risk elements in soils. Determination of polychlorinated biphenyls. 6. Toxicity test on the crustacean Daphnia magna. 7. Determination of inorganic and organic contaminants and pesticide residues in food, fruit and vegetables. 8. Determination of the dustiness of the working environment - respirable fraction, suspended particle fraction PM10, PM2.5, dust fallout, fibrogenic and fibrous dust. 9. - 10. Detection of pathogenic microbes. Determination of the number of microorganisms. Isolation, identification, confirmation. Biochemical tests.
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Learning activities and teaching methods
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- Preparation for an examination (30-60)
- 40 hours per semester
- Contact hours
- 60 hours per semester
- Preparation for laboratory testing; outcome analysis (1-8)
- 6 hours per semester
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| prerequisite |
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| Knowledge |
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| to explain the technique of taking different types of sample |
| Skills |
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| to master the physical and physico-chemical principles of analytical methods |
| Competences |
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| N/A |
| learning outcomes |
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| Knowledge |
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| characterizes the principles of analytical instrumentation selected for the needs of chemical investigation in the protection of public health |
| explains the principle of laboratory methods used in hygiene laboratories |
| Skills |
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| masters the methodology of determining pollutants in the living and working environment |
| controls sampling techniques of various matrices according to the specific requirements of specific analytes |
| performs physical measurements and measurements of chemical indicators, evaluates and interprets the results |
| Competences |
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| N/A |
| teaching methods |
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| Knowledge |
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| Lecture |
| Skills |
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| Laboratory work |
| Competences |
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| Laboratory work |
| assessment methods |
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| Knowledge |
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| Oral exam |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Oral exam |
| Skills demonstration during practicum |
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Recommended literature
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BAUDIŠOVÁ, D. Metody mikrobiologického rozboru vody: (příručka pro hydroanalytické laboratoře). 1. vyd.. Praha: Výzkumný ústav vodohospodářský T. G. Masaryka, 2017. ISBN 978-80-87402-61-0.
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DUDOVÁ, Jana. Právo na ochranu veřejného zdraví. Ochrana veřejného zdraví před rizikovými faktory venkovního prostředí. 1. vyd.. Praha: LINDE Praha, a.s., 2011. ISBN 978-80-7201-854-3.
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JIŘÍK, Vítězslav. Fyzikální a chemické laboratorní metody v ochraně veřejného zdraví.. Ostrava: Ostravská univerzita, Lékařská fakulta, 2018. ISBN 978-80-7599-007-5.
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KOTLÍK, B. a kol. Vzorkování II: životní prostředí.. Český Těšín: 2 Theta, 2016. ISBN 978-80-86380-81-0.
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PITTER, Pavel. Hydrochemie. 5., aktualizované a doplněné vydání.. Praha: Vysoká škola chemicko-technologická v Praze, 2015. ISBN 978-80-7080-928-0.
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ŘÍHOVÁ AMBROŽOVÁ, Jana, Dana VEJMELKOVÁ a Pavlína ČIHÁKOVÁ. Technická mikrobiologie a hydrobiologie. 1. vyd.. Praha: Vysoká škola chemicko-technologická v Praze, 2017. ISBN 978-80-7080-986-0.
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