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Lecturer(s)
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Course content
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A Lab exercise 1. Safety of work in the laboratory. Handling of equipment and chemicals, protective equipment, basics of metrology and ISO standards. 2. Laboratory glass and plastic. Laboratory supplies, classification of chemicals, measurement of liquids, weighing, preparation of solutions, filtration. 3. Laboratory equipment and basic procedures. Laboratory supplies in clinical laboratories, cooling and heating devices, laminar box, pipetting. 4. - 5. Types and preparation of biological material. Collection and types of biological material, preparation, isolation, homogenization. 6. - 8. Light, fluorescence and electron microscopy, preparation of specimens, microscopy. 9. - 11. Cell cultures. Cell isolation, cell viability, cell cultivation. 12. - 13. Analytical principles. Spectrophotometry, electrochemistry, immunochemistry. 14. - 15. Separation analytical methods. Electrophoresis, chromatography.
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Learning activities and teaching methods
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- Practical training (number of hours)
- 30 hours per semester
- Preparation for comprehensive test (10-40)
- 20 hours per semester
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| prerequisite |
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| Knowledge |
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| to demonstrate knowledge of high school biology and chemistry |
| Skills |
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| specific skill prerequisites are not required |
| Competences |
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| N/A |
| learning outcomes |
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| Knowledge |
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| will describe types of microscopic techniques |
| characterizes analytical principles in clinical laboratories |
| Skills |
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| has laboratory habits |
| operates basic laboratory equipment |
| works with biological material, including preparation |
| works with cell cultures |
| Competences |
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| N/A |
| teaching methods |
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| Knowledge |
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| Practicum |
| Skills |
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| Laboratory work |
| Competences |
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| Practicum |
| Laboratory work |
| assessment methods |
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| Knowledge |
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| Continuous assessment |
| Test |
| Skills |
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| Skills demonstration during practicum |
| Competences |
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| Continuous assessment |
| Test |
| Skills demonstration during practicum |
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Recommended literature
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DRECHSLEROVÁ, Taťána, PLAČKOVÁ, Tereza a Ilona BEČVÁŘOVÁ. Laboratorní technika.. Plzeň: Západočeská univerzita, 2015. ISBN 978-80-261-0533-6.
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SLABÝ, Ondřej. Lékařská biologie I: buněčná a molekulární biologie. 1. vydání.. Brno: Masarykova univerzita, 2022. ISBN 978-80-280-0060-8.
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ŠEBEK, Jaroslav. Buněčné kultury v medicíně.. Praha : Galén, 2018. ISBN 978-80-7492-380-7.
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ŠEBEK, Jaroslav. Hematologie a laboratorní technika.. Praha : Galén, 2022. ISBN 978-80-7492-631-0.
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ZIMA, Tomáš. Laboratorní diagnostika. Třetí, doplněné a přepracované vydání. Praha : Galén, 2013. ISBN 978-80-7492-062-2.
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