|
Lecturer(s)
|
-
Sirotek Vladimír, PaedDr. CSc.
-
Hrdlička Jan, Ing. Ph.D.
-
Štrofová Jitka, Mgr. Ph.D.
-
Richtr Václav, Doc. Mgr. CSc.
|
|
Course content
|
1st lecture Comparison of instrumental and chemical methods of analysis. Principles of high-performance liquid chromatography (HPLC). 2nd lecture HPLC instrumentation. LSC in polar adsorbents. LSC in nonpolar adsorbents. 3rd lecture Ion exchange chromatography. Gel permeation chromatography. Affinity chromatography. 4th lecture Affinity chromatography. Gas chromatography. Determination of polar and non-polar compounds. Principles of two-dimensional chromatographies. 5th lecture Ionization of the sampl in mass spectrometry. Instrumentation MS spectrometry. 6th lecture Interpretation of MS spectra. Use of the MS, GCMS and SIMS. 7th lecture Stripping voltammetry. Pulse polarography. Electroimpedance spectroscopy. 8th lecture Principle and instrumentation of atomic absorption spectroscopy. 9th lecture Applications AAS. Principle and the use of atomic fluorescence spectroscopy. 10th lecture Principle of the main methods of X-ray analysis. Instrumentation and the use of electron microanalysis. 11th lecture Instrumentation and the use of X-ray fluorescence analysis. Analytical use of X-ray difractometry. 12th lecture Spectroscopies of electrons. Principles and use. Exercises Polarography Gas Chromatography Polarimetry Spectrophotometry IR spectroscopy UV spectroscopy HPLC Potentiometry
|
|
Learning activities and teaching methods
|
Interactive lecture, Laboratory work, Self-study of literature
- Preparation for laboratory testing; outcome analysis (1-8)
- 8 hours per semester
- Preparation for an examination (30-60)
- 60 hours per semester
- Contact hours
- 36 hours per semester
|
| prerequisite |
|---|
| Knowledge |
|---|
| to describe basic principles of instrumental methods |
| to describe basic procedures for qualitative chemical analysis |
| to describe basic procedures for titration and gravimetric assays |
| formulate relationships for basic chemical equilibria |
| Skills |
|---|
| use direct instrumental determination using calibration |
| evaluate graph data |
| learning outcomes |
|---|
| Knowledge |
|---|
| to list the basic electrochemical, optical and separative methods |
| to define their properties and uses |
| to describe the data you collect and find out the information you need |
| Skills |
|---|
| to explain the choice of the method for the desired determination |
| to work with common analytical instruments |
| teaching methods |
|---|
| Knowledge |
|---|
| Interactive lecture |
| Laboratory work |
| Self-study of literature |
| assessment methods |
|---|
| Combined exam |
|
Recommended literature
|
-
BALCI M. Basic 1H- and 13C-NMR Spectroscopy. ProQuest Ebook Central. Elsevier Science & Technology, 2005.
-
Barek, Jiří; Opekar, František,; Štulík, Karel. Elektroanalytická chemie. 1. vyd. Praha : Karolinum, 2005. ISBN 80-246-1146-5.
-
KATZ E., EKSTEEN R., SCHOENMAKERS P., MILLER N. Handbook of HPLC. Marcel Dekker Inc, New York, 1998. ISBN 0-8247-9444-3.
-
Němcová Irena; Engst Pavel; Jelínek Ivan; Sejbal Jan; Rychlovský Petr. Spektrometrické analytické metody II. Praha, 1998. ISBN 80-7184-586-8.
-
Němcová, Irena; Rychlovský, Petr; Čermáková, Ludmila. Spektrometrické analytické metody I. 2. vyd. Praha : Karolinum, 2004. ISBN 80-246-0776-X.
-
SCHRADER B. Infrared and Raman spectroscopy : methods and applications. John Wiley & Sons, Incorporated, 2008. ISBN 978-3-527-26446-9.
-
SKÁCEL, F., TEKÁČ, V. Analýza ovzduší. VŠCHT, Praha, 2019. ISBN 978-80-7592-038-6.
-
SKOOG, H. A., HOLLER, F. J., CROUCH, S. R. Principles of Instrumental Analysis, Cengage Learning. 2017. ISBN 978-1-305-57721-3.
-
Štulík, Karel. Analytické separační metody. 1. vyd. Praha : Karolinum, 2004. ISBN 80-246-0852-9.
-
WANG J. Analytical Electrochemistry. ProQuest Ebook Central. John Wiley & Sons, Incorporated, 2006.
|