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Course info
KAZ / PKL1
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Course description
Department/Unit / Abbreviation
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KAZ
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PKL1
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Academic Year
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2023/2024
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Academic Year
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2023/2024
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Title
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Practice in clinical lab 1 (Mi,Hi,Im)
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Form of course completion
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Pre-Exam Credit
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Form of course completion
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Pre-Exam Credit
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Long Title
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Practice in clinical laboratory 1 (Microbiology,Histopathology,Imunology)
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Accredited / Credits
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Yes,
9
Cred.
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Type of completion
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Combined
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Type of completion
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Combined
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Time requirements
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Practice
240
[Hours/Semester]
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Course credit prior to examination
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No
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Course credit prior to examination
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No
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Automatic acceptance of credit before examination
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Yes in the case of a previous evaluation 4 nebo nic.
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Included in study average
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NO
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Language of instruction
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Czech
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Occ/max
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Automatic acceptance of credit before examination
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Yes in the case of a previous evaluation 4 nebo nic.
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Summer semester
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0 / -
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0 / -
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0 / -
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Included in study average
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NO
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Winter semester
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0 / -
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0 / -
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0 / -
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Repeated registration
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NO
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Repeated registration
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NO
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Timetable
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Yes
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Semester taught
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Summer semester
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Semester taught
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Summer semester
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Minimum (B + C) students
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10
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Optional course |
No
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Optional course
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No
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Language of instruction
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Czech
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Internship duration
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30
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No. of hours of on-premise lessons |
0
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Evaluation scale |
S|N |
Periodicity |
každý rok
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Periodicita upřesnění |
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Fundamental theoretical course |
No
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Fundamental course |
Yes
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Fundamental theoretical course |
No
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Evaluation scale |
S|N |
Substituted course
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None
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Preclusive courses
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N/A
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Prerequisite courses
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KAZ/ZBMN
and KAZ/MIL1
and KAZ/HI
and KAZ/HT and KAZ/IMUN1
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Meet all prerequisites before registering
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NO
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Informally recommended courses
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N/A
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Courses depending on this Course
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N/A
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Histogram of students' grades over the years:
Graphic PNG
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XLS
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Course objectives:
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Students practically carry out selected tasks and activities and become more familiar with the organization of operation and basic documentation of laboratory workplaces. They improve in basic microbiological examinations and techniques that are used in medical microbiological laboratories, in basic histological techniques in the processing of a biological tissue sample and in the determination of antibodies in immunological laboratories.
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Requirements on student
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100% attendance
Confirmation of completion of practice by the workplace and evaluation by a mentor in Logbook.
Processing of the selected method and its presentation at the Student Conference.
Elaboration of an essay in which the student describes the operation of the relevant laboratory, his involvement in routine operation and critically evaluates which of the activities he has improved and which may not have been without possible errors.
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Content
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MICROBIOLOGY - 2 weeks (80 hours)
Content content of practice
- operating rules of the relevant microbiological laboratory, principles of work safety, principles of disinfection and sterilization.
- receipt and identification of material, SOP
- preparation of cultivation media and possibly sterility verification
- examination of the throat, nose, swabs from the ear, conjunctival sac
- examination of wounds
- examination of sputum, bronchial lavage of pus
- examination of urine, urethral swabs
- stool examination
- examination of dialysate, punctate
- cerebrospinal fluid examination
- examination of cannula, catheter
- blood examination from blood culture
- anaerobic examination
- mycological examination
- examination of part of the tissue
- examination for Helicobacter pylori from a biopsy sample
- examination of the vaginal image
- examination of mycoplasma, ureaplasma
- Chlamydia examination
- sterility examination
- determining the minimum inhibitory concentration of the antibiotic and allowing bound antibiotics
- examination of Legionella pneumophila
HISTOPATHOLOGY - 2 weeks (80 hours)
Content content of practice
- preparation of material for histological use
- basic freezing technique
- the technique of making microtome sections
- hematoxylin - eosin staining
- Mallory staining
- histochemistry of inorganic substances
- Feulgen reaction, methyl green, apyronin
- PAS, Best's crimson
- staining of mucopolysaccharides, mucoproteins and glycoproteins
- sudanophilia, OsO4
- enzyme staining
- epithelial tissue, connective tissue, muscle tissue, nervous system, circulatory system, respiratory system, lymphatic system, glands with internal secretion, digestive system, urinary system, sexual system, skin, and nervous system, senses
IMMUNOLOGY - 2 weeks (80 hours)
Content content of practice
- internal and external quality control of examinations in the immunological laboratory, sampling of material
- ELISA, RIA
- immunofluorescence methods, IgA, IgE
- CRP
- antibodies against grasses
- antibodies against milk and gliacin
- antibodies against mites
- complement
- immunocomplexes
- determination of T lymphocytes
- opsonization test
- NBT test
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Activities
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Fields of study
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Guarantors and lecturers
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Literature
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-
Basic:
JIRKOVSKÁ, Marie. Histologická technika: základy. Druhé, doplněné vydání.. Praha : Galén, 2017. ISBN 978-80-7492-325-8.
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Basic:
VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ a Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody. 1. vyd.. Brno: Neptun, 2010. ISBN 978-80-86850-04-7.
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Basic:
BARTŮŇKOVÁ, Jiřina, PAULÍK, Milan a kol. Vyšetřovací metody v imunologii. 2., přepracované a doplněné vydání.. Praha : Grada, 2011. ISBN 978-80-247-3533-7.
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Recommended:
VOTAVA, Miroslav. Lékařská mikrobiologie II.: přehled vyšetřovacích metod v lékařské mikrobiologii.. Brno: Masarykova univerzita, 2000. ISBN 80-210-2272-8.
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On-line library catalogues
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Time requirements
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Full-time form of study
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Activities
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Time requirements for activity [h]
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Presentation preparation (report) (1-10)
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5
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Practical training (number of hours)
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240
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Total
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245
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Prerequisites
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Knowledge - students are expected to possess the following knowledge before the course commences to finish it successfully: |
to describe the microanatomical structure of tissues, organs and organ systems |
to describe methods of collecting histological material and explain the technique of processing a sample of tissue or cells for microscopic analysis |
to explain the procedure for processing hard tissues for histological examination |
to characterize individual types of staining |
to describe the types of staining techniques in the microbiology laboratory |
to explain the principles of laboratory methods for monitoring humoral and cellular immunity |
to explain the principle of ELISA immunochemical methodology using an enzyme to mark an antigen or an antibody |
Skills - students are expected to possess the following skills before the course commences to finish it successfully: |
to classify individual tissues |
independently to perform fixation, embedding, staining and sectioning of tissues |
to collect and transport a biological sample to the microbiology laboratory |
to observe health protection principles when handling a biological sample |
to determine the method of species identification of microorganisms (light microscopy, cultivation, biochemical, immunological and molecular methods) |
to perform protein detection under supervision, including Ig nephelometry and turbidimetry and detection of circulating immunocomplexes |
by the flow cytometry method to determine under supervision the phenotyping of T and B lymphocytes, the basophil activation test, blast transformation of lymphocytes and oxidative burst |
Competences - students are expected to possess the following competences before the course commences to finish it successfully: |
N/A |
N/A |
N/A |
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Learning outcomes
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Knowledge - knowledge resulting from the course: |
defines the content of the laboratory manual, operating and hygiene regulations of the microbiological, histopathological and immunological laboratory |
Skills - skills resulting from the course: |
applies the principles of aseptic work in the microbiology laboratory in order to prevent contamination of samples with live microbial cells during laboratory processing |
evaluates the external and internal quality of the microbiological examination of the collected biological material |
processes the collected sample from a technical and methodological point of view and describes it |
determines sensitivity to ATB |
prepares biological material for histological use, processes it, cuts and stains it |
masteres basic freezing technique and work on a microtome |
determines antibodies, number and differential budget of leukocytes in peripheral blood |
works with a flow cytometer, turbidimeter or nephelometer and controls instrumentation for performing and evaluating ELISA reactions |
prepares preparations for immunofluorescence examination |
Competences - competences resulting from the course: |
N/A |
N/A |
N/A |
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Assessment methods
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Knowledge - knowledge achieved by taking this course are verified by the following means: |
Continuous assessment |
Skills - skills achieved by taking this course are verified by the following means: |
Skills demonstration during practicum |
Individual presentation at a seminar |
Competences - competence achieved by taking this course are verified by the following means: |
Skills demonstration during practicum |
Continuous assessment |
Individual presentation at a seminar |
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Teaching methods
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Knowledge - the following training methods are used to achieve the required knowledge: |
Clinical practice under supervision |
Skills - the following training methods are used to achieve the required skills: |
Clinical practice under supervision |
Competences - the following training methods are used to achieve the required competences: |
Clinical practice under supervision |
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